Adelaide forward Mitch McGovern has failed in his bid to play in the Grand Final against Richmond on Saturday at the MCG.
McGovern completed about 45 minutes of the Crows' 90-minute open training session at Adelaide Oval on Wednesday morning before skipping the last couple drills.
The 22-year-old needed to get through the whole session to prove his fitness from a hamstring injury that forced him to miss the Preliminary Final.
"Unfortunately, he won't come up, it's an opportunity for someone else," Crows coach Don Pyke said.
Crows chief executive Andrew Fagan added: "It's a sad story for 'Gov', but one man down, another one up."
Andy Otten is the favourite to keep his spot in the side, while Wayne Milera Juior will also be considered.
"Match committee will meet on Thursday, they will make their determination," Fagan said.
"It's not just about Andy; there are a bunch of guys on the fringes who have played well throughout the course of the year.
"There is one game to go, it's going to be tough decision for match committee."
McGovern completed the warm-up with the main group, which included running at half pace and short sprints.
He was then able to take part in general field kicking and didn't seem to lack any power, launching a couple of huge bombs close to 50m.
But about 45 minutes into the session, McGovern didn't take part in a drill with the forwards and was instead having a discussion with assistant coach Tate Kaesler.
McGovern then watched on as the Crows did a full-ground drill to replicate a game situation.
In better news, midfielder Hugh Greenwood got through the majority of the session despite speculation he is carrying a calf injury.
Greenwood played just 59 per cent of game time and had just six possessions in the Preliminary Final against Geelong.
More than 10,000 Crows fans flocked to the open training session, the last chance they will get to see their heroes before they aim to win the Club's first premiership since 1998.
The biggest cheer was reserved for champion forward Eddie Betts when he ran on to the ground.
BREAKING: Mitch McGovern has been ruled out of this Saturday's #AFLGF. More to come on https://t.co/s7LxURFFSp #weflyasone pic.twitter.com/aUMpSm5vgJ
— Adelaide Crows (@Adelaide_FC) September 27, 2017